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Journal: Cell metabolism

Article Title: NAD+ regeneration rescues lifespan but not ataxia in a mouse model of brain mitochondrial complex I dysfunction

doi: 10.1016/j.cmet.2020.06.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RNA was extracted using a QIAGEN RNeasy Plus Micro Kit following manufacturer protocol and qPCR was carried out using a CYBRFast 1-step RT-qPCR Lo-Rox Kit (Tonbo Biosciences) following manufacturer protocol with a BioRad CFX384 Real Time System. qPCR Primers: NDUFS4 forward 5’-GAGCACATCCACTTGGAAGC NDUFS4 reverse 5’-GATGTGCTCTTCTGGAACACC NDI1 forward 5’-GCCGAAGAAGTCCAAATTCAC NDI1 reverse 5’-CGACAGCCGTTCTCAGAT Actin forward 5’-CTAAGGCCAACCGTGAAAAG Actin reverse 5’-ACCAGAGGCATACAGGGACA NADH/NAD+ Ratio NADH/NAD+ ratio was determined on enriched astrocyte primary cultures from post-natal mouse cerebellum using the NAD/NADH Glo Assay (Promega) following the manufacturer-supplied protocol.

Techniques: Recombinant, Western Blot, Glo Assay, Construct, Software, Imaging